Unknown Bacteria

INTRODUCTION
This section introduces the reader to the study and why the study was done. This should only be a
few sentences long.
Example: “There are many reasons for knowing the identity of microorganisms. The
reasons range from the knowing the causative agent of a disease in a patient, so as to know how it
can be treated, to knowing the correct microorganism to be used for making certain foods or
antibiotics. This study was done by applying all of the methods that have been learned so far in the
microbiology laboratory class for the identification of an unknown bacterium.”
MATERIALS AND METHODS
This is where the details of the study are listed. Where did the specimen come from, and what
methods were used to identify it? Be specific, but do not re write the lab manual. One way is to
mention the names of the materials used and reference the lab manual for the procedure or method
and then continue to elaborate when necessary. See example 1.
Example 1: “An unknown labeled as letter G was given out by the lab instructor. The
methods that have been learned thus far for identifying bacteria have been applied to this unknown.
Procedures were followed as stated in the course laboratory manual by De Mers (1), unless
otherwise noted.
The first procedure that needed to be done was to streak the unknown out on a Trypticase Soy Agar
plate, using the T streak method described in the lab manual.. This needed to be done in order to test
the purity of the unknown. After the plates were incubated and grown, the morphology was
observed and recorded and a Gram stain was performed. Quality control bacteria were Gram stained
along with the unknown to make sure that the Gram stain reaction was done correctly . After
determining the Gram reaction, specific biochemical tests were performed. The biochemical tests
were chosen from the unknown identification tables that were in the lab manual. Since unknown G
was determined to be a Gram negative rod, an oxidase test was performed and the organism was
inoculated into a BCP lactose tube. Note all of these tests were performed by the methods listed in
the lab manual by De Mers (1). Table 1 lists the test, purpose, reagents and results. All of the
following tests were performed on this unknown:
1. Oxidase test
2. BCP Lactose
3. Indole
4. H2S
5. Citrate
6. Motility
7. Methyl Red
8. Urea”

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