Covid-19 is actually an RNA virus. Discuss what extra step(s) you need to do in order for your PCR based detection of the virus to be successful


Go to the primer design website and register for an account:

Primer design website:

(free after registration)


Exercise:  download the Covid-19 N protein nucleotide sequence here:



copy the sequence into the website and design your own primers against Covid-19.  Select regular PCR (PCR – 2 primers).  You may leave all other parameters at the default settings.

Select any set of primers from the website.  Click on any of the primer sets and click “assay details”  and do a printout of the screen showing the sequence of the forward primer, reverse primer,  and the sequence of the Covid-19 N protein and save that as a PDF file.


Upload the PDF file together with your assignment.   You may also just copy and paste that page directly into this word file.


After you have chosen the primers, copy and paste the sequence of the primer into the NEB website to determine what’s the best enzyme Annealing temperature determination for your PCR reaction:!/main


You may choose any of the commonly used PCR enzymes (I personally prefer Taq or Vent but others would work too).  Note the annealing temperature would be different depending on what enzymes you are using.


Save the NEB screen showing the the Tm of the primers, the enzyme you have chosen and the suggested annealing temperature


(20 points for the pictures)





Answer ALL of the following questions:


  1. Write out all the steps you need to do your own PCR test as follows:
  2. All Reagents needed: (5 points)








  1. Temperature programming of the thermo cycler for the primers and enzyme you have chosen. (10 points)







  1. Comments on what may happen if you set the annealing temperature (a) too low and (b) too high. (5 points)






  1. Covid-19 is actually an RNA virus. Discuss what extra step(s) you need to do in order for your PCR based detection of the virus to be successful    (5 points)







  1. In a hypothetical PCR process, primer 1 has Tm of 55ºC and primer 2 has Tm of 60º Discuss what annealing temperature you would set your PCR machine for the PCR reaction to be successful.  Provide a brief explanation of your choice   (5 points)


Get 20% Discount on This Paper
Pages (550 words)
Approximate price: -

Try it now!

Get 20% Discount on This Paper

We'll send you the first draft for approval by at
Total price:

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

Quality Custom Writers has assembled a team of highly skilled writers with diverse experience in the online writing circles. Our aim is to become a one stop shop for all your Academic/ online writing. Check out below our amazing service!


Essay Writing Services

At Quality Custom Writers, we prioritize on all aspects that creates a good grade such as impeccable grammar, proper structure, zero-plagiarism, and conformance to guidelines. The principal purpose of essay writing is to present the author's evaluation concerning a singular subject about which they have made. Since Professionalism is the mother of every success, try our team of experienced writers in helping you complete your essays and other assignments.


Admission Papers

You have been trying to join that prestigious institution you long yearned for, but the hurdle of an admission essay has become a stumbling block. We have your back, with our proven team that has gained invaluable experience over time, your chance of joining that institution is now! Just let us work on that essay.How do you write an admission essay? How do you begin the essay? For answers, try Quality Custom Writers Now!


Editing and Proofreading

Regardless of whether you're pleased with your composing abilities, it's never an impractical notion to have a second eye go through your work. The best editing services leaves no mistake untouched. We recognize the stuff needed to polish up a writing; as a component of our editing and proofreading, we'll change and refine your write up to guarantee it's amazing, and blunder free. Our group of expert editors will examine your work, giving an impeccable touch of English while ensuring your punctuation and sentence structures are top-notch.


Technical papers

We pride ourselves in having a team of clinical writers. The stringent and rigorous vetting process ensures that only the best persons for job. We hire qualified PhD and MA writers only. We equally offer our team of writers bonuses and incentives to motivate their working spirit in terms of delivering original, unique, and informative content. They are our resources drawn from diverse fields. Therefore your technical paper is in the right hands. Every paper is assessed and only the writers with the technical know-how in that field get to work on it.


College Essay Writing

If all along you have been looking for a trustworthy college essay service provider that provides superb academic papers at reasonable prices, then be glad that you search has ended with us. We are your best choice! Get high-quality college essay writing from our magnificent team of knowledgeable and dedicated writers right now!


Quality Assignment/Homework Help

We give the students premium quality assignments, without alarming them with plagiarism and referencing issues. We ensure that the assignments stick to the rules given by the tutors. We are specific about the deadlines you give us. We assure you that you will get your papers well in advance, knowing that you will review and return it if there are any changes, which should be incorporated.

× How can I help you?